Dr. Shobha Anilkumar

Biosketch
Dr. Shobha Anilkumar’s journey started at the Human Brain Tissue Repository (HBTR) at the Department of Neuropathology, NIMHANS, where she was instrumental in setting up the Human Brain Bank, a first of its kind, under the guidance of Dr. S. K. Shankar. Apart from this, she was also involved in projects investigating the spread of HERPES SIMPLEX VIRUS in the human brain. Through this project, she gained hands-on experience working with human tissue and developed expertise in a range of histological techniques while working at the Department of Neuropathology, NIMHANS.
She then joined the National Centre for Biological Sciences (NCBS). At NCBS she was part of Prof. Sumantra Chattarjis’s (Shona) research group. With her expertise in the field of histology, she set up a morphological facility at Shona’s laboratory to study stress-induced structural plasticity in rodent brains. She standardized and simplified the techniques of Golgi-Cox staining that was widely used as a simple histology protocol. While working at NCBS, she registered for her PhD degree and received a PhD from Manipal Institute of Higher Education (MAHE) where her thesis was titled “Stress-Induced Plasticity in Limbic Circuits.”
She joined CBR in 2019 as a Scientific Officer. She manages the operational activities of Biobank at CBR alongside her research interests in structural plasticity underlying neurodegenerative diseases, particularly Alzheimer’s Disease and Parkinson’s Disease.
Projects:
Non-research activities:
Dr. Shobha Anilkumar is the scientific officer in charge of biobank operations, including Quality Management System services (QMS). She played a prominent role in the ISO20387 accreditation process right from the preparation of Standard Operating Procedures (SOPs), drafting of the Biobank manual according to ISO standards, staff assessment, and training. CBR Biobank received its accreditation on the 8th th of August 2024.
She manages the operations of the CBR Biobank by closely coordinating with other scientific officers and faculty members involved in CBR’s flagship research projects. Her key responsibilities include overseeing the procurement process, ensuring timely maintenance, and handling the annual renewal of maintenance contracts for all critical equipment. She regularly monitors the stock of consumables (reagents, kits, and plasticware), conducts periodic inventory checks, and places indents with the purchasing department to maintain optimal stock levels. Additionally, she manages and oversees blood collection camps in Srinivaspura, organizes logistics, and coordinates with local staff to ensure smooth operations. She oversees scheduling for blood collection under projects like the Tata Longitudinal Study of Aging (TLSA) and GenomeIndia, ensuring optimal utilization of resources and personnel. Routine monitoring of freezer temperatures and updating of logbooks are part of her duties to maintain biobank freezer performance and preserve sample integrity. In collaboration with other scientific officers and faculty members, she prioritizes blood samples for DNA isolation, sequencing, APOE genotyping, and plasma biomarker analysis. She also maintains the records of assay results, providing timely information to faculty members involved in various flagship projects upon request.
She manages the operations of the CBR Biobank by closely coordinating with other scientific officers and faculty members involved in CBR’s flagship research projects. Her key responsibilities include overseeing the procurement process, ensuring timely maintenance, and handling the annual renewal of maintenance contracts for all critical equipment. She regularly monitors the stock of consumables (reagents, kits, and plasticware), conducts periodic inventory checks, and places indents with the purchasing department to maintain optimal stock levels. Additionally, she manages and oversees blood collection camps in Srinivaspura, organizes logistics, and coordinates with local staff to ensure smooth operations. She oversees scheduling for blood collection under projects like the Tata Longitudinal Study of Aging (TLSA) and GenomeIndia, ensuring optimal utilization of resources and personnel. Routine monitoring of freezer temperatures and updating of logbooks are part of her duties to maintain biobank freezer performance and preserve sample integrity. In collaboration with other scientific officers and faculty members, she prioritizes blood samples for DNA isolation, sequencing, APOE genotyping, and plasma biomarker analysis. She also maintains the records of assay results, providing timely information to faculty members involved in various flagship projects upon request.
Research activities :
Research Focus on Vascular Dementia and Parkinson’s Disease:
Her interests are towards elucidating how the dendritic and spinal morphology are affected in the neurodegenerative diseases (using transgenic mouse models) like Alzheimer’s and Parkinson’s diseases and vascular dementia.
She works in collaboration with the laboratory of Dr. Latha Diwakar, Senior Scientific Officer, CBR.
Dr. Diwakar’s lab is exploring two major areas of neurodegenerative disease research. The first being vascular dementia and the other project being Parkinson disease progression. Dr. Anilkumar contributes to both projects with her expertise in histological and morphological techniques, aiming to provide insights into the pathology of vascular dementia and Parkinson’s disease progression.
The first project investigates how endothelin peptide, which induces Transient Ischemic Attacks (TIAs), affects neuronal morphology and leads to cognitive impairment in vascular dementia (mouse models). The second project focuses on understanding motor and non-motor symptoms in a Parkinson’s disease (PD) transgenic mouse model. Using a comprehensive approach involving Golgi Cox staining and immunohistochemical markers, the team assesses dendritic architecture and spine density to unravel the cellular mechanisms underlying these conditions. Dr. Anilkumar also monitors the functioning of the microscopy and histology facility.
Lab Members:
Mohan C
Senior Lab Assistant
Rajesh G
Lab Assistant
Divakara A
Lab Technician
Rakesh KH
Lab Technician
Recurrent endothelin-1 mediated vascular insult leads to cognitive impairment protected by trophic factor pleiotrophin. Pushpam M, Talukdar A, Anilkumar S, Maurya SK, Issac TG, Diwakar L. Exp Neurol. 2024 Nov;381:114938. doi:10.1016/j.expneurol.2024.114938. Epub 2024 Aug 26. PMID: 39197707
Longitudinally Divergent Dendritic Spine densities of the Hippocampus and Amygdala:
An Impact of Aggressor-Exposed Social Stress. Book Chapter Horizons in Neuroscience Research, Vol,46, Chapter 4; .page no:113-144
Shobha Anilkumar, Nabarun Chakraborty, James Meyerhoff, Ross Campbell, Seid Muhie, Rasha Hammamieh and Marti Jett
Decreased dendritic spine density in posterodorsal medial amygdala neurons of proactive coping rats. Anilkumar S, Patel D, de Boer SF, Chattarji S, Buwalda B.Behav Brain Res. 2021 Jan 15;397:112940. doi: 10.1016/j.bbr.2020.112940. Epub 2020 Oct 27.PMID: 33126115
Repeated victorious and defeat experiences induce similar apical dendritic spine remodeling in CA1 hippocampus of rats. Patel D, Anilkumar S, Chattarji S, de Boer SF, Buwalda B.
Repeated social stress leads to contrasting patterns of structural plasticity in the amygdala and hippocampus. Patel D, Anilkumar S, Chattarji S, Buwalda B. Behav Brain Res. 2018 Jul 16;347:314-324. doi: 10.1016/j.bbr.2018.03.034. Epub 2018 Mar 23. PMID: 29580891
Stress enhances fear by forming new synapses with greater capacity for long-term potentiation in the amygdala. Suvrathan A, Bennur S, Ghosh S, Tomar A, Anilkumar S, Chattarji S.Philos Trans R Soc Lond B Biol Sci. 2013 Dec 2;369(1633):20130151. doi: 10.1098/rstb.2013.0151. Print 2014 Jan 5.
Genetic and acute CPEB1 depletion ameliorate fragile X pathophysiology. Udagawa T, Farny NG, Jakovcevski M, Kaphzan H, Alarcon JM, Anilkumar S, Ivshina M, Hurt JA,Nagaoka K, Nalavadi VC, Lorenz LJ, Bassell GJ, Akbarian S, Chattarji S, Klann E, Richter JD. Nat Med. 2013 Nov;19(11):1473-7. doi: 10.1038/nm.3353. Epub 2013 Oct 20.
The same antidepressant elicits contrasting patterns of synaptic changes in the amygdala vs hippocampus. Pillai AG, Anilkumar S, Chattarji S. Neuropsychopharmacology. 2012 Nov;37(12):2702-11. doi: 10.1038/npp.2012.135. Epub 2012 Jul 25.
Disruption of fatty acid amide hydrolase activity prevents the effects of chronic stress on anxiety and amygdalar microstructure. Hill MN, Kumar SA, Filipski SB, Iverson M, Stuhr KL, Keith JM, Cravatt BF, Hillard CJ, Chattarji S, McEwen BS.Mol Psychiatry. 2013 Oct;18(10):1125-35. doi: 10.1038/mp.2012.90. Epub 2012 Jul 10.
Glucocorticoids protect against the delayed behavioral and cellular effects of acute stress on the amygdala. Rao RP, Anilkumar S, McEwen BS, Chattarji S. Biol Psychiatry. 2012 Sep 15;72(6):466-75. doi: 10.1016/j.biopsych.2012.04.008. Epub 2012 May 8.
Expression of nestin-a stem cell associated intermediate filament in human CNS tumours. Rani SB, Mahadevan A, Anilkumar SR, Raju TR, Shankar SK . Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Bangalore, India. Indian 2006 Sep;124(3):269-80
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Centre for Brain Research
Indian Institute of Science Campus
CV Raman Avenue
Bangalore 560012, India. - linkedin.com